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Fluorometer MFLM-1C
Model: MFLM-1C · Category: Fluorometer
Fluorometer MFLM-1C is the next generation of benchtop dual-channel Fluorometer, features 460 ± 20 nm and 525 ± 20 nm excitation filter and measures dsDNA concentration with sensitivity of 5 ng/ul. It uses blue and green channel fluorescence to measure fluorescence intensity of fluorescent reagent that combines with target molecule to read concentration of sample. Designed to accurately detect high-sensitive fluorescence while quantifying DNA, RNA, and protein.
Specifications
| Dynamic range | Five orders of magnitude |
|---|---|
| Linear dynamic range | R > 0.995 |
| Processing time | ≤3 seconds/sample |
| Light sources | Blue LED Green LED |
| Excitation filters | Blue 460±20 nm Green 525±20 nm |
| Emission filters | Blue 525 to 570 nm Green 575 to 640 nm |
| Detector | Photodiode: measurement capability from 300 to 1000 nm |
| Repeatability | <1.5% |
| Stability | <1.5% |
| Sensitivity | dsDNA: 0.5 ng/ml |
| Dimension (W×D×H) | 194 × 155 × 72.5 mm |
| weight | 0.4 kg |
Features
| Simple operation with 4.3-inch touch screen, small and easy to use |
| Accurately quantifies DNA, RNA, and protein in <3 seconds per sample |
| High sensitivity with lowest DNA detection limit of 0.5 ng/ul |
| Two optical channels equipped with two fluorescence channels for macromolecule quantitation in one detection |
| Blue and green dual fluorescence channels |
| Linear dynamic range with five orders of magnitude |
| It can save at most 1,000 data and output data by USB port |
Applications
Ideal for processing scarce and difficult samples, for low quantity of DNA, RNA, or protein after extraction, also, in expensive experiments: qPCR, PCR cloning, transfection, the next generation sequencing and etc. Applied for Rhodamine detection, Cy-3 fluorescence labeling detection, RFP gene detection, Cell activity position detection.
Standard Accessories
| Accessories no | Accessories name |
|---|---|
| 1. | Adapter for 0.5ml qPCR tube |
Optional Accessories
| Accessories no | Accessories name |
|---|---|
| 1. | Adapter for 0.2ml qPCR tube |
| 2. | Printer |
Fluorescence Channels
| Blue and green channels | |
| For blue fluorescence channel: | |
| Light source | Blue LED |
|---|---|
| Excitation wavelength | 460±20 nm |
| Emission wavelength | 525 to 570 nm (45 nm) |
| For green fluorescence channel: | |
| Light source | green LED |
| Excitation wavelength | 525±20 nm |
| Emission wavelength | 575 to 640 nm (65 nm) |
